Previous research has shown that Schiff bases have a stimulating effect on the production of the
antibiotic Azalomycine B by the bacterium Streptomyces higroscopicus. Also, it was confirmed that
waste glycerol obtained in biodiesel production from vegetable and inedible oils can be the common
starting material for valuable industrial products such as antibiotics. In this paper, the results of
studying the possibility of combining isatin derivatives of benzoyl hydrazone as a nitrogen source
with waste glycerol, obtained in the rapeseed oil-based biodiesel production, as a carbon source for
manufacturing antibiotic Azalomycine B by S. hygroscopicus CH-7 strain are presented. Depending
on the nitrogen source (benzoyl hydrazone derivatives), the achieved maximal concentrations of
azalomycine B range from 33 to 50 μg/ml, and the biomass content range from 5.5 to 5.9 g/l. The
highest maximal concentration of Azalomycine B (50 μg/ml) and the specific rate of production (1.3
μg/g/d) were achieved in the medium with 5-chloroisatin-3 (4`hidroxy) benzoylhydrasone. In general,
the main difference in the structure of these compounds is the substituent at position 5, and chlorine
in this case did not have a negative effect on antibiotic production.
