Nuclear magnetic resonance spectroscopy (NMR) is wide spread method for
investigation of organic compounds structure including polyphenols. For the
identification of polyphenols, the most common NMR technique include one –
dimensional NMR techniques such as 1H and 13C. However, to overcome problems
of spectral overlap two-dimensional nuclear magnetic resonance spectroscopy (2D
NMR) is used. 2D NMR is a set of NMR methods that gives data plotted in a space
defined by two frequency axes rather than one. The most popular two-dimension
NMR experiments are the homonuclear correlation spectroscopy (COSY)
sequence, heteronuclear single-quantum correlation spectroscopy (HSQC) and
heteronuclear two-bond or multiple-bond correlation spectroscopy (H2BC, HMBC,
respectively). In this study, results of eight different polyphenol compounds
structure determined by advanced 2D-NMR technique (COSY, HSQC, H2BC, and
HMBC) are shown.
