Аутор и коаутори: Jelena Nikitović, Tatjana Pandurević, Marko Lazić

TOTAL BILIRUBIN CONCENTRATION AND AST ACTIVITY IN SERUM OF SIMMENTAL COWS IN DIFFERENT GEOGRAPIC REGIONS OF BOSNIA AND HERZEGOVINA

Година објаве: 2016

Језик: Енглески

Сажетак:

The objective of this study was to evaluate serum bilirubin concentration and AST activity in Simmental cows in two different geographic regions (Lijevce polje lowlands and high altitude Nevesinje). A total of 42 healthy cows, 21 per farm, were tested. Cows were allocated to three groups viz. late pregnant or dry cows (7 animals), cows in the first days post-calving (7 animals), and cows in the second to third month of lactation (7 animals). The study was conducted during the autumn-winter period (2008) and repeated during spring (2009) involving the same cows. Blood samples were collected for analysis by puncture of the coccygeal vein (vena coccigea) using sterile needles into sterile single-use vacuette blood collection tubes without an anticoagulant. After 24 hour storage at room temperature, serum was separated, deep-frozen, and transported within 48 days to the laboratory for further biochemical assays. Blood (serum) bilirubin concentration was within the physiological range in all experimental cows in both locations. The results related to AST activity showed a slight increase above the physiological limit in all groups of cows, with late pregnant cows (Nevesinje location, winter period) exhibiting significantly higher values. Key words: Concentration, Serum, Geographic area, Bilirubin, AST