Surveys for TSWV presence and distribution
on vegetable, ornamental and tobacco plants were conducted
during 2016 in open fields and greenhouses in
different locations in Republic of Srpska, district of
Bosnia and Herzegovina. One hundred and twelve samples
were taken and preliminarily tested for TSWV presence
with DAS-ELISA. Positive samples were doublechecked
in RT-PCR assays using specific primer sets and
subsequently partial sequences of RdRp and N region
genes were characterized. Serological and molecular results
revealed twenty-seven TSWV positive samples (two
peppers and twenty-five tobacco plants). Moreover, phylogenetic
analyses showed that these isolates share the
same gene pool and a similar evolutionary pattern mainly
with other isolates present in Europe and Russia
