Introduction. High-risk human papilloma viruses play a main role
in the development of cervical dysplasias and carcinomas.
p16INK4a can be considered as a surrogate marker of active highrisk human papillomaviruses infection in dysplastic and neoplastic
cells of the cervix. This study was aimed at determining the presence and level of p16INK4a expression in inflammatory, preneoplastic and neoplastic lesions of the cervix. Material and Methods.
The study was performed on 109 samples of cervical biopsy. Cervical cancer was diagnosed in 36 patients, 34 patients had a preneoplastic change (dysplasia) in stratified squamous cervix epithelium
and a nonspecific inflammatory process was found in 39 patients.
In all samples, immunohistochemical analysis using antibodies to
p16INK4a was performed. Results. The expression of p16INK4a
was verified in all cases of cervical cancer (100%), in 67.65% of
dysplastic cervical lesions and in 38.5% of inflammatory lesions.
A statistically highly significant difference was found in the presence and level of expression among neoplasic, dysplastic and inflammatory lesions of the cervix (χ² = 76.02, p < 0.001). The expression was more frequent and had a higher level in neoplastic and
high grade dysplastic lesions compared to expression in inflammatory lesions and low grade dysplasias. Conclusion. The analysis of
the presence of p16INK4a can differentiate non-neoplastic, high
grade preneoplastic and neoplastic changes of the cervix. The use
of p16INK4a in interpreting borderline lesions of the cervix can
enable a rational therapeutic treatment of patients
